anti wnt2  (Proteintech)

Journal: Pharmacology Research & Perspectives

Article Title: Lobaric Acid Exhibits Anticancer Potential by Modulating the Wnt/β‐Catenin Signaling Pathway in MCF‐7 Cells

doi: 10.1002/prp2.70142

Figure Lengend Snippet: Effect of LA on Wnt/β‐catenin pathway in MCF‐7 cells. (A) The migration of cells treated with LA into the wound was visualized at 0, 6, 12, and 24 h under an inverted microscope, and the percentage of wound closure was evaluated statistically. (B–I) Expressions of WNT2 , DVL1 , AXIN1 , β‐Catenin , TCF‐4 , CCND1 , c‐MYC , and CDK1 genes analyzed by qPCR in LA‐treated MCF‐7 cells at 48 h. (J–M) Protein expressions of WNT2, GSK3‐ β, and β‐catenin determined by Western blot analysis. The experiment was performed in three biological and technical replicates. * p < 0.05, ** p < 0.01, and *** p < 0.001 in relation to the control. Scale bar, 500 μm.

Article Snippet: Subsequently, membrane incubation was performed overnight at 4°C using P53 antibody (Proteintech, 10442‐1‐AP, 1:1000), BCL2 antibody (Proteintech 12789‐1‐AP 1:500), WNT2 antibody (Proteintech, 66656‐I‐Ig, 1:1000), AXIN antibody (Proteintech, 68093‐1‐Ig, 1:1000), β‐Catenin antibody (Santa Cruz Biotechnology, sc‐7963, 1:1000), GSK3‐ β antibody (Santa Cruz, sc‐377213, 1:1000), and β‐Actin antibody (Santa Cruz Biotechnology, sc‐47778, 1:1000).

Techniques: Migration, Inverted Microscopy, Western Blot, Control

Journal: PeerJ

Article Title: Anti-inflammatory role of low-intensity pulsed ultrasound in inhibiting lipopolysaccharide-induced M1 polarization of RAW264.7 cells via Wnt2b/AXIN/β-catenin

doi: 10.7717/peerj.18448

Figure Lengend Snippet: The relative expression of Wnt2b, one of the key proteins in the Wnt signaling pathway, was detected by RT-qPCR in RAW264.7 cells, during treatment with (A) LIPUS and LPS or (B) LIPUS and IL4. RAW indicates RAW264.7 cells used as control. RAW-LPS indicates RAW264.7 cells treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS indicates RAW264.7 cells treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LIPUS indicates RAW264.7 cells treated with low-intensity pulsed ultrasound. RAW+IL4 indicates RAW264.7 cells treated with 20 ng/mL IL4 to induce macrophage-like M2 polarization. RAW+IL4+LIPUS indicates RAW264.7 cells treated with 20 ng/mL IL4 and low-intensity pulsed ultrasound. * P < 0.05, ** P < 0.005, **** P < 0.0001.

Article Snippet: Primary antibodies used in the study included Wnt2b (66656-1-Ig; Proteintech, CA, Rosemont, IL, USA), AXIN (20540-1-AP; Proteintech, Rosemont, Illinois, China), β-catenin (51067-2-AP; Proteintech, Rosemont, IL, USA), and GAPDH (10494-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: PeerJ

Article Title: Anti-inflammatory role of low-intensity pulsed ultrasound in inhibiting lipopolysaccharide-induced M1 polarization of RAW264.7 cells via Wnt2b/AXIN/β-catenin

doi: 10.7717/peerj.18448

Figure Lengend Snippet: (A) CD80 and (B) CD86 were detected by flow cytometry during M1 polarization of RAW264.7 cells. RAW+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA, used as control. RAW+LPS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LPS+LIPUS+Wnt2bsi1/2/3 indicates RAW264.7 cells transfected with Wnt2b siRNA and treated with low-intensity pulsed ultrasound. *** P < 0.0005, **** P < 0.0001.

Article Snippet: Primary antibodies used in the study included Wnt2b (66656-1-Ig; Proteintech, CA, Rosemont, IL, USA), AXIN (20540-1-AP; Proteintech, Rosemont, Illinois, China), β-catenin (51067-2-AP; Proteintech, Rosemont, IL, USA), and GAPDH (10494-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Flow Cytometry, Transfection, Negative Control, Control

Journal: PeerJ

Article Title: Anti-inflammatory role of low-intensity pulsed ultrasound in inhibiting lipopolysaccharide-induced M1 polarization of RAW264.7 cells via Wnt2b/AXIN/β-catenin

doi: 10.7717/peerj.18448

Figure Lengend Snippet: Relative expression of (A) Wnt2b, (B) AXIN, and (C) β-catenin detected by Western blot. RAW-Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA, used as control. RAW+LPS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LPS+LIPUS+Wnt2bsi1/2/3 indicates RAW264.7 cells transfected with Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. **** P < 0.0001.

Article Snippet: Primary antibodies used in the study included Wnt2b (66656-1-Ig; Proteintech, CA, Rosemont, IL, USA), AXIN (20540-1-AP; Proteintech, Rosemont, Illinois, China), β-catenin (51067-2-AP; Proteintech, Rosemont, IL, USA), and GAPDH (10494-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing, Western Blot, Transfection, Negative Control, Control

Journal: PeerJ

Article Title: Anti-inflammatory role of low-intensity pulsed ultrasound in inhibiting lipopolysaccharide-induced M1 polarization of RAW264.7 cells via Wnt2b/AXIN/β-catenin

doi: 10.7717/peerj.18448

Figure Lengend Snippet: (A) The expression of IL2, IL4, IL6, IL10, TNFα, IFNγ, and IL17A were detected by flow cytometry. (B) The expression of IL6 and TNFα was increased by the downregulation of Wnt2b during LIPUS and LPS treatment. RAW+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA, used as control. RAW+LPS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LPS+LIPUS+Wnt2bsi1/2/3 indicates RAW264.7 cells transfected with Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. * P < 0.05, **** P < 0.0001.

Article Snippet: Primary antibodies used in the study included Wnt2b (66656-1-Ig; Proteintech, CA, Rosemont, IL, USA), AXIN (20540-1-AP; Proteintech, Rosemont, Illinois, China), β-catenin (51067-2-AP; Proteintech, Rosemont, IL, USA), and GAPDH (10494-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing, Flow Cytometry, Transfection, Negative Control, Control

Journal: Cell reports

Article Title: Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration

doi: 10.1016/j.celrep.2023.112647

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-WNT2 , proteintech , Cat# 27214-1-ap ; RRID: AB_2880804.

Techniques: Recombinant, Staining, SYBR Green Assay, Red Blood Cell Lysis, Protease Inhibitor, cDNA Synthesis, Generated, Transgenic Assay, Software